Cell Architecture
Presented by Karl Johnson ; version 7.0 release (2014)

An Advanced On-Line Cell Structure Course/Text

2014 Meeting Dates
October 28

Compartmentation of the Eukaryotic Cell

October 28

The Nucleus

October 30
Bone, Courtney R., Erin C. Tapley, Mátyás Gorjánácz, and Daniel A. Starr. “The Caenorhabditis Elegans SUN Protein UNC-84 Interacts with Lamin to Transfer Forces from the Cytoplasm to the Nucleoskeleton during Nuclear Migration.Molecular Biology of the Cell 25, no. 18 (September 15, 2014): 2853–65. doi:10.1091/mbc.E14-05-0971.
October 30

The Organelles: Mitochondria and Chloroplasts

November 4
Davies, K, M Strauss, B Daum, J Kief, H Osiewacz, A Rycovska, V Zickermann, and W Kuhlbrandt. “Macromolecular Organization of ATP Synthase and Complex I in Whole Mitochondria.” Proceedings of the National Academy of Sciences 108, no. 34 (November 20, 2011): 14121. doi:10.1073/pnas.1103621108.
November 4

The Endomembrane System

November 6

Chao, Jesse T., Andrew K. O. Wong, Shabnam Tavassoli, Barry P. Young, Adam Chruscicki, Nancy N. Fang, LeAnn J. Howe, Thibault Mayor, Leonard J. Foster, and Christopher J. R. Loewen. “Polarization of the Endoplasmic Reticulum by ER-Septin Tethering.” Cell 158, no. 3 (July 31, 2014): 620–32. doi:10.1016/j.cell.2014.06.033.

Organization of the Eukaryotic Cell

November 6


November 11
Breitsprecher, D, R Jaiswal, J Bombardier, C Gould, J Gelles, and B Goode. “Rocket Launcher Mechanism of Collaborative Actin Assembly Defined by Single-Molecule Imaging.” Science 336, no. 6085 (December 20, 2012): 1164. doi:10.1126/science.1218062.
November 11


November 13
Ali, M, G Kennedy, D Safer, K Trybus, H Sweeney, and D Warshaw. “Myosin Va and Myosin VI Coordinate Their Steps While Engaged in an in Vitro Tug of War during Cargo Transport.” Proceedings of the National Academy of Sciences 108, no. 34 (November 20, 2011): E535. doi:10.1073/pnas.1104298108.
November 13


November 18
Preitner, Nicolas, Jie Quan, Dan W. Nowakowski, Melissa L. Hancock, Jianhua Shi, Joseph Tcherkezian, Tracy L. Young-Pearse, and John G. Flanagan. “APC Is an RNA-Binding Protein, and Its Interactome Provides a Link to Neural Development and Microtubule Assembly.” Cell 158, no. 2 (July 17, 2014): 368–82. doi:10.1016/j.cell.2014.05.042.
November 18


November 20
McKenney, Richard J., Walter Huynh, Marvin E. Tanenbaum, Gira Bhabha, and Ronald D. Vale. “Activation of Cytoplasmic Dynein Motility by Dynactin-Cargo Adapter Complexes.” Science 345, no. 6194 (July 18, 2014): 337–41. doi:10.1126/science.1254198.
November 20


November 25
Tanaka, K, Y Sugiura, R Ichishita, K Mihara, and T Oka. “KLP6: A Newly Identified Kinesin That Regulates the Morphology and Transport of Mitochondria in Neuronal Cells.Journal of Cell Science, 2011. doi:10.1242/jcs.086470.

Functionality and the Eukaryotic Cell

November 25


December 2
Nguyen, Phuong A., Aaron C. Groen, Martin Loose, Keisuke Ishihara, Martin Wühr, Christine M. Field, and Timothy J. Mitchison. “Spatial Organization of Cytokinesis Signaling Reconstituted in a Cell-Free System.” Science 346, no. 6206 (October 10, 2014): 244–47. doi:10.1126/science.1256773.
December 2

Asymmetric Cell Division, Cell Polarity and Development

December 4
Williams, Scott, Slobodan Beronja, H. Amalia Pasolli, and Elaine Fuchs. “Asymmetric Cell Divisions Promote Notch-Dependent Epidermal Differentiation.” Nature 470, no. 7334 (February 17, 2011): 353–58. doi:10.1038/nature09793.

The Eukaryotic Cell and its Environment

December 4

Cell-Cell Connections

December 9
Kanchanawong, Pakorn, Gleb Shtengel, Ana M. Pasapera, Ericka B. Ramko, Michael W. Davidson, Harald F. Hess, and Clare M. Waterman. “Nanoscale Architecture of Integrin-Based Cell Adhesions.” Nature 468, no. 7323 (November 25, 2010): 580–84. doi:10.1038/nature09621.
December 9

The Extracellular Matrix

December 11
Ott, Harald, Thomas Matthiesen, Saik-Kia Goh, Lauren Black, Stefan Kren, Theoden Netoff, and Doris Taylor. “Perfusion-Decellularized Matrix: Using Nature’s Platform to Engineer a Bioartificial Heart.” Nature Medicine 14, no. 2 (August 20, 2008): 213. doi:10.1038/nm1684.
December 11



Cell Architecture WWW

Cell Architecture is presented by Karl Johnson, Haverford College, Pennsylvania

This page last updated 12/05/2014

Each chapter of this e-text is a compedium of information, images and other open source materials gathered to help you explore the eukaryotic cell and is followed by an interactive discussion of a recent paper that is influencing the way we look at the cell from structure/function perspectives.

Cell Architecture debuted at Haverford College in 1993-1994 and first ventured on the World Wide Web in 1994-1995 (using the "first" web browser, NCSA Mosaic 0.53b). It subsequently has grown through several major rebuilds and is continuously edited, modified and updated as ideas grow and change (it is, after all, biological!). While I have tried to make this text a stand-alone resource freely available to all, there is no substitute for coming to Haverford and joining our class. Visitors, virtual or real, are always welcomed!

Sources from which images are cached are available below the accompanying commentary text, and almost every image and video was downloaded from other open-access, open-source sites; our effort is largely collecting and making sense of it all. Our sincere thanks to everyone who makes their work available on the Internet for teaching purposes, and it is in reciprocity that we hope you enjoy this site. We do not claim copyright for any of this material; we have simply cached and organized freely accessible material providing a link to its source (based on the model of a web search engine). This approach was necessary to provide a stable resource in the fluid environment of the Web. Known issues: videos shown in-class do not play in the lecture notes (we are working on this!).

Literature links: All article links point directly to the corresponding journal web site, and so anyone visiting this page will be able to access articles to which their institution has arranged library access. This should be seamless and transparent for most users, including Haverford, Bryn Mawr and Swarthmore students on their campus networks. For Haverford students who travel off-campus, articles access is possible by using our campusVPN service or ezproxy.haverford.edu.

Finally, for students in the class, biweekly question sets will form the basis for your evaluation in this course. The question sets will be distributed to members of the class a week before the due date by link to the table below. Each will consist of several open exploration questions and students are expected to use the resources of the web, particularly the scientific literature, in forming their responses. Completed question sets should be submitted electronically as .doc or .docx files as e-mail attachments to Karl Johnson . As always, back-up work in progress and retain a copy of your submission file until your work is graded and returned. Early submissions are welcomed; late work will be accepted but will be marked down a grade for each day (24 hour period) it is late based on submission times recorded by the e-mail server. Each student's grade in the course will be based upon these question sets (@20%) and a class attendance/participation mark (40%).

Question Set
Distributed by
Due (Midnight of)
Saturday, 11/1/2014
Saturday, 11/8/2014
Saturday, 11/22/2014
Wednesday, 12/3/2014
Saturday, 12/6/2014
Saturday, 12/13/2014

In December, 2014, Philadelphia is hosting the annual American Society for Cell Biology Meeting in the downtown convention center. There is a special program for undergraduate students on Saturday, Dec. 6th, that involves a Graduate School Fair, several presentations, and a poster session. Registration is free but must be submitted in advance using this form. This is a great opportunity to learn about graduate programs and experience the excitement of a large, professional meeting literally at our doorstep. Please consider attending; to encourage participation, Cell Architecture students who do so may submit a two page synopsis of their experience at the meeting in place of their third question set.

I hope you enjoy Cell Architecture! Please feel free to contact me with suggestions and comments!